edu staining assay Search Results


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Edu Staining Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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edu staining proliferation kit  (Ribobio co)
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Edu Staining Proliferation Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Cell Light Edu Staining Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Edu Immuno Uorescence Staining Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Edu Staining Solution, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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edu staining of uterine slides  (Ribobio co)
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Edu Staining Of Uterine Slides, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
One Step Edu Staining Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Edu Staining Solution, supplied by U.S Everbright, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
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edu staining assay cell-lighttm apollo 488 stain kit  (Ribobio co)
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IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell <t>proliferation</t> assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.
Edu Staining Assay Cell Lighttm Apollo 488 Stain Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell proliferation assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Journal: Investigative Ophthalmology & Visual Science

Article Title: m 6 A-Mediated Upregulation of Imprinted in Prader–Willi Syndrome Induces Aberrant Apical–Basal Polarization and Oxidative Damage in RPE Cells

doi: 10.1167/iovs.65.2.10

Figure Lengend Snippet: IPW overexpression in RPE cells induces oxidative damage. ( A ) ROS levels detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( B ) Gene set enrichment analysis (GSEA) plot of oxidative stress pathway in fRPE cells overexpressing IPW. ( C ) Cell viability detected by CCK8 assay in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( D ) Cell proliferation assessed by EdU assay in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( E ) GSEA plot shows negative regulation of epithelial cell proliferation pathway in fRPE cells overexpressing IPW. ( F ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with indicated lentivirus (n = 3 per group). Grey area (left) indicates for G1 phase; purple area (middle) indicates for S phase; orange area (right) indicates for G2 phase. ( G ) Apoptosis detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW (n = 3 per group). ( H ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells transduced with indicated lentivirus (n = 3 per group). ( I ) GO plot of enriched apoptosis-related pathways in fRPE cells overexpressing IPW. Data are presented as mean ± SEM. Two-tailed Student t test was used. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Proliferation of fRPE cells was assessed using an EdU staining proliferation kit (RiboBio) according to the manufacturer's protocol.

Techniques: Over Expression, Transduction, CCK-8 Assay, EdU Assay, Western Blot, Two Tailed Test

Rapamycin suppresses the AKT/mTOR pathway to alleviate the IPW-induced oxidative damage. ( A ) ROS accumulation detected by flow cytometric analyses in fRPE cells receiving indicated treatments (n = 3 per group). ( B ) Cell proliferation detected by EdU assay in fRPE cells receiving distinct treatments (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( C ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW and treated with or without rapamycin (n = 3 or 4 per group). Grey area ( left ) indicates for G1 phase; purple area ( middle ) indicates for S phase; orange area ( right ) indicates for G2 phase. ( D ) Apoptosis detected by flow cytometric analyses in fRPE cells receiving indicated treatments (n = 3 per group). ( E ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells receiving distinct treatments (n = 3 per group). Data are presented as mean ± SEM. One-way ANOVA coupled with the Bonferroni's post hoc test was used. NS, not significant ( P > 0.05), * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Investigative Ophthalmology & Visual Science

Article Title: m 6 A-Mediated Upregulation of Imprinted in Prader–Willi Syndrome Induces Aberrant Apical–Basal Polarization and Oxidative Damage in RPE Cells

doi: 10.1167/iovs.65.2.10

Figure Lengend Snippet: Rapamycin suppresses the AKT/mTOR pathway to alleviate the IPW-induced oxidative damage. ( A ) ROS accumulation detected by flow cytometric analyses in fRPE cells receiving indicated treatments (n = 3 per group). ( B ) Cell proliferation detected by EdU assay in fRPE cells receiving distinct treatments (n = 3 per group). Cell nuclei are counterstained with DAPI. Scale bar, 60 µm. ( C ) Cell cycle progression detected by flow cytometric analyses in fRPE cells transduced with L-EV or L-IPW and treated with or without rapamycin (n = 3 or 4 per group). Grey area ( left ) indicates for G1 phase; purple area ( middle ) indicates for S phase; orange area ( right ) indicates for G2 phase. ( D ) Apoptosis detected by flow cytometric analyses in fRPE cells receiving indicated treatments (n = 3 per group). ( E ) Immunoblotting of caspase-3, cleaved caspase-3, Bax/Bcl-2 in fRPE cells receiving distinct treatments (n = 3 per group). Data are presented as mean ± SEM. One-way ANOVA coupled with the Bonferroni's post hoc test was used. NS, not significant ( P > 0.05), * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Proliferation of fRPE cells was assessed using an EdU staining proliferation kit (RiboBio) according to the manufacturer's protocol.

Techniques: EdU Assay, Transduction, Western Blot